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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become noticed in many tumors, which leads to chromosome instability, and therefore might add to tumorigenesis. This protein was discovered to affiliate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle controlled histone gene expression and performs a important role in advertising cell-cycle development in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, have already been described. Two more splice variants had been described but comprehensive nucleotide sequence details isn't nevertheless out there.
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Blocking peptides are peptides that bind particularly to your focus on antibody and block antibody binding. These peptide typically has the epitope identified from the antibody.
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This information outlines further attainable optimization of mobile and immunoassay parameters to ensure the very best benefits are...
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This guide outlines additional attainable optimization of cellular and immunoassay parameters to make sure the absolute best benefits are...
Antibodies certain to the blocking peptide no more bind on the epitope over the concentrate on protein. By evaluating the staining with the blocked antibody vs . the antibody alone, you can see which staining more info is unique.
Concentrate on Information and facts Cyclin E belongs into the highly conserved cyclin family members, whose members are characterized by a spectacular periodicity in protein abundance throughout the mobile cycle. Cyclins perform as regulators of CDK kinases. Distinct cyclins show distinct expression and degradation styles which contribute to the temporal coordination of each and every mitotic function. Cyclin E forms a fancy with and capabilities for a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S transition. This protein accumulates in the G1-S period boundary which is degraded as cells progress through S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by the two antibodies...
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